A quantitative atlas of mitotic phosphorylation
Harvard University · Brigham and Women's Hospital · +1 more institution
Abstract
The eukaryotic cell division cycle is characterized by a sequence of orderly and highly regulated events resulting in the duplication and separation of all cellular material into two newly formed daughter cells. Protein phosphorylation by cyclin-dependent kinases (CDKs) drives this cycle. To gain further insight into how phosphorylation regulates the cell cycle, we sought to identify proteins whose phosphorylation is cell cycle regulated. Using stable isotope labeling along with a two-step strategy for phosphopeptide enrichment and high mass accuracy mass spectrometry, we examined protein phosphorylation in a human cell line arrested in the G(1) and mitotic phases of the cell cycle. We report the…
Citation impact
- FWCI
- 53.75
- Percentile
- 100%
- References
- 42
Authors
7Topics & keywords
- Cyclin-dependent kinase
- Phosphorylation
- Mitosis
- Cell cycle
- Protein phosphorylation
- Phosphopeptide
- Biology
- Cell biology