articleJournal of the American Chemical SocietyMar 21, 2014GREEN OA

Cyanine-Based Fluorescent Probe for Highly Selective Detection of Glutathione in Cell Cultures and Live Mouse Tissues

JYJun YinYKYounghee KwonDKDabin KimDLDa-Young LeeGKGyoungmi Kim

Ewha Womans University · Central China Normal University · +1 more institution

PubMed
Indexed incrossrefpubmed

Abstract

Glutathione (GSH) plays a crucial role in human pathologies. Near-infrared fluorescence-based sensors capable of detecting intracellular GSH in vivo would be useful tools to understand the mechanisms of diseases. In this work, two cyanine-based fluorescent probes, 1 and 2, containing sulfonamide groups were prepared. Evaluation of the fluorescence changes displayed by probe 1, which contains a 2,4-dinitrobenzenesulfonamide group, shows that it is cell-membrane-permeable and can selectively detect thiols such as GSH, cysteine (Cys), and homocysteine (Hcy) in living cells. The response of 1 to thiols can be reversed by treatment with N-methylmaleimide (NMM). Probe 2, which possesses a…

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Topics & keywords

Topics
Keywords
  • Glutathione
  • Chemistry
  • Cyanine
  • Fluorescence
  • In vivo
  • Intracellular
  • Cysteine
  • Biochemistry
UN Sustainable Development Goals
  • Good health and well-being
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