Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting

Wiklander, Oscar P. B.; Nordin, Joel Z.; O’Loughlin, Aisling J.; Gustafsson, Ylva; Corso, Giulia; Mäger, Imre; Vader, Pieter; Lee, Yi; Sork, Helena; Seow, Yiqi; Heldring, Nina; Alvarez‐Erviti, Lydia; Smith, CI Edvard; Blanc, Katarina Le; Macchiarini, Paolo; Jungebluth, Philipp; Wood, Matthew J. A.; Andaloussi, Samir EL

doi:10.3402/jev.v4.26316

articleJournal of Extracellular VesiclesJan 1, 2015GOLD OA

Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting

OPOscar P. B. Wiklander JZJoel Z. Nordin AJAisling J. O’Loughlin YGYlva Gustafsson GCGiulia Corso

Karolinska Institutet · University of Oxford · +9 more institutions

PubMed

Indexed incrossrefdoajpubmed

Abstract

Extracellular vesicles (EVs) have emerged as important mediators of intercellular communication in a diverse range of biological processes. For future therapeutic applications and for EV biology research in general, understanding the in vivo fate of EVs is of utmost importance. Here we studied biodistribution of EVs in mice after systemic delivery. EVs were isolated from 3 different mouse cell sources, including dendritic cells (DCs) derived from bone marrow, and labelled with a near-infrared lipophilic dye. Xenotransplantation of EVs was further carried out for cross-species comparison. The reliability of the labelling technique was confirmed by sucrose gradient fractionation, organ perfusion and further…

Citation impact

1,650

total citations

FWCI: 30.27
Percentile: 100%
References: 56

Citations per year

Authors

18

Topics & keywords

Topics

Keywords

Biodistribution
In vivo
Spleen
Cell biology
Extracellular vesicle
Ex vivo
Biology
Vesicle

No related works found for this paper.