MicroRNAs Regulate Brain Morphogenesis in Zebrafish
University of North Carolina at Chapel Hill · Wellcome Sanger Institute · +4 more institutions
Abstract
MicroRNAs (miRNAs) are small RNAs that regulate gene expression posttranscriptionally. To block all miRNA formation in zebrafish, we generated maternal-zygotic dicer (MZdicer) mutants that disrupt the Dicer ribonuclease III and double-stranded RNA-binding domains. Mutant embryos do not process precursor miRNAs into mature miRNAs, but injection of preprocessed miRNAs restores gene silencing, indicating that the disrupted domains are dispensable for later steps in silencing. MZdicer mutants undergo axis formation and differentiate multiple cell types but display abnormal morphogenesis during gastrulation, brain formation, somitogenesis, and heart development. Injection of miR-430 miRNAs rescues the brain defects…
Citation impact
- FWCI
- 43.38
- Percentile
- 100%
- References
- 40
Authors
9- AJAntonio J. GiráldezCorresponding
University of North Carolina at Chapel Hill, Wellcome Sanger Institute, Whitehead Institute for Biomedical Research, UNC Lineberger Comprehensive Cancer Center, New York University, Massachusetts Institute of Technology
- RMRyan M. Cinalli
University of North Carolina at Chapel Hill, Wellcome Sanger Institute, Whitehead Institute for Biomedical Research, UNC Lineberger Comprehensive Cancer Center, New York University, Massachusetts Institute of Technology
- MEMargaret E. Glasner
University of North Carolina at Chapel Hill, Wellcome Sanger Institute, Whitehead Institute for Biomedical Research, UNC Lineberger Comprehensive Cancer Center, New York University, Massachusetts Institute of Technology
- AJAnton J. Enright
University of North Carolina at Chapel Hill, Wellcome Sanger Institute, Whitehead Institute for Biomedical Research, UNC Lineberger Comprehensive Cancer Center, New York University, Massachusetts Institute of Technology
- JMJ. Michael Thomson
University of North Carolina at Chapel Hill, Wellcome Sanger Institute, Whitehead Institute for Biomedical Research, UNC Lineberger Comprehensive Cancer Center, New York University, Massachusetts Institute of Technology
Topics & keywords
- Zebrafish
- Morphogenesis
- microRNA
- Biology
- Cell biology
- Fish <Actinopterygii>
- Neuroscience
- Computational biology