Macrophage‐dependent clearance of systemically administered B16BL6‐derived exosomes from the blood circulation in mice
Kyoto University · Kyoto Pharmaceutical University
Abstract
Previous studies using B16BL6-derived exosomes labelled with gLuc-lactadherin (gLuc-LA), a fusion protein of Gaussia luciferase (a reporter protein) and lactadherin (an exosome-tropic protein), showed that the exosomes quickly disappeared from the systemic circulation after intravenous injection in mice. In the present study, the mechanism of rapid clearance of intravenously injected B16BL6 exosomes was investigated. gLuc-LA-labelled exosomes were obtained from supernatant of B16BL6 cells after transfection with a plasmid DNA encoding gLuc-LA. Labelling was stable when the exosomes were incubated in serum. By using B16BL6 exosomes labelled with PKH26, a lipophilic fluorescent dye, it was demonstrated that…
Citation impact
- FWCI
- 13.25
- Percentile
- 100%
- References
- 25
Authors
9- TITakafumi ImaiCorresponding
Kyoto University, Kyoto Pharmaceutical University
- YTYuki Takahashi
Kyoto University, Kyoto Pharmaceutical University
- MNMakiya Nishikawa
Kyoto University, Kyoto Pharmaceutical University
- KKKana Kato
Kyoto University, Kyoto Pharmaceutical University
- MMMasaki Morishita
Kyoto University, Kyoto Pharmaceutical University
Topics & keywords
- Microvesicles
- Exosome
- Spleen
- Macrophage
- Transfection
- Molecular biology
- Chemistry
- Immunology