Structural Characterization of Flexible Proteins Using Small-Angle X-ray Scattering
Institut de Biologie Structurale · Université Joseph Fourier · +7 more institutions
Abstract
Structural analysis of flexible macromolecular systems such as intrinsically disordered or multidomain proteins with flexible linkers is a difficult task as high-resolution techniques are barely applicable. A new approach, ensemble optimization method (EOM), is proposed to quantitatively characterize flexible proteins in solution using small-angle X-ray scattering (SAXS). The flexibility is taken into account by allowing for the coexistence of different conformations of the protein contributing to the experimental scattering pattern. These conformers are selected using a genetic algorithm from a pool containing a large number of randomly generated models covering the protein configurational space. Quantitative…
Citation impact
- FWCI
- 14.93
- Percentile
- 100%
- References
- 41
Authors
5- PBPau BernadóCorresponding
Institut de Biologie Structurale, Université Joseph Fourier, European Molecular Biology Laboratory, Russian Academy of Sciences, Université Grenoble Alpes, CEA Grenoble, European Molecular Biology Laboratory, Centre National de la Recherche Scientifique, Commissariat à l'Énergie Atomique et aux Énergies Alternatives
- EMEfstratios Mylonas
CEA Grenoble, Université Joseph Fourier, European Molecular Biology Laboratory, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Université Grenoble Alpes, European Molecular Biology Laboratory, Russian Academy of Sciences, Centre National de la Recherche Scientifique, Institut de Biologie Structurale
- MVMaxim V. Petoukhov
European Molecular Biology Laboratory, Université Grenoble Alpes, European Molecular Biology Laboratory, Russian Academy of Sciences, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Université Joseph Fourier, Centre National de la Recherche Scientifique, Institut de Biologie Structurale, CEA Grenoble
- MBMartin Blackledge
European Molecular Biology Laboratory, Université Joseph Fourier, CEA Grenoble, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Russian Academy of Sciences, European Molecular Biology Laboratory, Institut de Biologie Structurale, Université Grenoble Alpes, Centre National de la Recherche Scientifique
- DIDmitri I. Svergun
Université Joseph Fourier, European Molecular Biology Laboratory, CEA Grenoble, European Molecular Biology Laboratory, Institut de Biologie Structurale, Centre National de la Recherche Scientifique, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Université Grenoble Alpes, Russian Academy of Sciences
Topics & keywords
- Small-angle X-ray scattering
- Conformational isomerism
- Chemistry
- Scattering
- Macromolecule
- Conformational ensembles
- Intrinsically disordered proteins
- Flexibility (engineering)