Structures of the Bacterial Ribosome at 3.5 A Resolution
Lawrence Berkeley National Laboratory · Institute of Crystallography · +1 more institution
Abstract
70S ribosome with a global resolution of 2.0 Å. The maps reveal unambiguous positioning of protein and RNA residues, their detailed chemical interactions, and chemical modifications. Notable features include the first examples of isopeptide and thioamide backbone substitutions in ribosomal proteins, the former likely conserved in all domains of life. The maps also reveal extensive solvation of the small (30S) ribosomal subunit, and interactions with A-site and P-site tRNAs, mRNA, and the antibiotic paromomycin. The maps and models of the bacterial ribosome presented here now allow a deeper phylogenetic analysis of ribosomal components including structural conservation to the level of solvation. The high…
Citation impact
- FWCI
- 43.59
- Percentile
- 100%
- References
- 100
Authors
7- BSB.S. SchuwirthCorresponding
Lawrence Berkeley National Laboratory, Institute of Crystallography, University of California, Berkeley
- MBM.A. BorovinskayaCorresponding
Lawrence Berkeley National Laboratory, Institute of Crystallography, University of California, Berkeley
- CHC.W. Hau
Lawrence Berkeley National Laboratory, Institute of Crystallography, University of California, Berkeley
- WZWen Zhang
Lawrence Berkeley National Laboratory, Institute of Crystallography, University of California, Berkeley
- AVAntón Vila‐Sanjurjo
Lawrence Berkeley National Laboratory, Institute of Crystallography, University of California, Berkeley
Topics & keywords
- Ribosome
- Ribosomal RNA
- Transfer RNA
- 30S
- Eukaryotic Ribosome
- Protein subunit
- 50S
- Angstrom