articleACS Synthetic BiologyFeb 5, 2014Closed access

A Golden Gate Modular Cloning Toolbox for Plants

CECarola EnglerMYMark YoulesRGRamona GruetznerTETim-Martin EhnertSWStefan Werner

Sainsbury Laboratory · Norwich Research Park · +1 more institution

PubMed
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Abstract

Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versatile resource for plant biologists comprising a set of cloning vectors and 96 standardized parts to enable Golden Gate construction of multigene constructs for plant transformation. Parts include promoters, untranslated sequences, reporters, antigenic tags, localization signals, selectable markers, and terminators. The comparative performance of parts in the model plant Nicotiana benthamiana is discussed.

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Topics & keywords

Topics
Keywords
  • Golden gate
  • Nicotiana benthamiana
  • Cloning (programming)
  • Transformation (genetics)
  • Selectable marker
  • Computational biology
  • Synthetic biology
  • Modular design
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