Escherichia coli Induces DNA Double-Strand Breaks in Eukaryotic Cells
Centre National de la Recherche Scientifique · Institut Pasteur · +4 more institutions
Abstract
Transient infection of eukaryotic cells with commensal and extraintestinal pathogenic Escherichia coli of phylogenetic group B2 blocks mitosis and induces megalocytosis. This trait is linked to a widely spread genomic island that encodes giant modular nonribosomal peptide and polyketide synthases. Contact with E. coli expressing this gene cluster causes DNA double-strand breaks and activation of the DNA damage checkpoint pathway, leading to cell cycle arrest and eventually to cell death. Discovery of hybrid peptide-polyketide genotoxins in E. coli will change our view on pathogenesis and commensalism and open new biotechnological applications.
Citation impact
- FWCI
- 6.95
- Percentile
- 100%
- References
- 28
Authors
10- JNJean‐Philippe Nougayrède
Centre National de la Recherche Scientifique, Institut Pasteur, École Nationale Vétérinaire de Toulouse, University of Würzburg, Universitätsmedizin Göttingen, University of Göttingen
- SHStefan Homburg
Centre National de la Recherche Scientifique, Institut Pasteur, École Nationale Vétérinaire de Toulouse, University of Würzburg, Universitätsmedizin Göttingen, University of Göttingen
- FTFrédéric Taïeb
Centre National de la Recherche Scientifique, Institut Pasteur, École Nationale Vétérinaire de Toulouse, University of Würzburg, Universitätsmedizin Göttingen, University of Göttingen
- MBMichèle Boury
Centre National de la Recherche Scientifique, Institut Pasteur, École Nationale Vétérinaire de Toulouse, University of Würzburg, Universitätsmedizin Göttingen, University of Göttingen
- EBElżbieta Brzuszkiewicz
Centre National de la Recherche Scientifique, Institut Pasteur, École Nationale Vétérinaire de Toulouse, University of Würzburg, Universitätsmedizin Göttingen, University of Göttingen
Topics & keywords
- Escherichia coli
- Biology
- Polyketide
- DNA
- DNA damage
- Nonribosomal peptide
- G2-M DNA damage checkpoint
- Gene
- Life below water