Age‐related intrinsic changes in human bone‐marrow‐derived mesenchymal stem cells and their differentiation to osteoblasts
Brigham and Women's Hospital · Harvard University · +2 more institutions
Abstract
In vivo and in vitro studies indicate that a subpopulation of human marrow-derived stromal cells (MSCs, also known as mesenchymal stem cells) has potential to differentiate into multiple cell types, including osteoblasts. In this study, we tested the hypothesis that there are intrinsic effects of age in human MSCs (17-90 years). We tested the effect of age on senescence-associated beta-galactosidase, proliferation, apoptosis, p53 pathway genes, and osteoblast differentiation in confluent monolayers by alkaline phosphatase activity and osteoblast gene expression analysis. There were fourfold more human bone MSCs (hMSCs) positive for senescence-associated beta-galactosidase in samples from older than younger…
Citation impact
- FWCI
- 15.18
- Percentile
- 100%
- References
- 59
Authors
7- SZShuanhu ZhouCorresponding
Brigham and Women's Hospital, Harvard University
- JSJoel S. Greenberger
University of Pittsburgh, University of Pittsburgh Medical Center
- MWMichael W. Epperly
University of Pittsburgh, University of Pittsburgh Medical Center
- JPJulie P. Goff
University of Pittsburgh, University of Pittsburgh Medical Center
- CECarolyn E. Adler
Brigham and Women's Hospital, Harvard University
Topics & keywords
- Mesenchymal stem cell
- Osteoblast
- Senescence
- Biology
- Alkaline phosphatase
- Stem cell
- Stromal cell
- Bone marrow
- Clean water and sanitation