Human 3D vascularized organotypic microfluidic assays to study breast cancer cell extravasation
Massachusetts Institute of Technology · Istituto Ortopedico Galeazzi · +4 more institutions
Abstract
A key aspect of cancer metastases is the tendency for specific cancer cells to home to defined subsets of secondary organs. Despite these known tendencies, the underlying mechanisms remain poorly understood. Here we develop a microfluidic 3D in vitro model to analyze organ-specific human breast cancer cell extravasation into bone- and muscle-mimicking microenvironments through a microvascular network concentrically wrapped with mural cells. Extravasation rates and microvasculature permeabilities were significantly different in the bone-mimicking microenvironment compared with unconditioned or myoblast containing matrices. Blocking breast cancer cell A3 adenosine receptors resulted in higher extravasation rates…
Citation impact
- FWCI
- 19.54
- Percentile
- 100%
- References
- 44
Authors
7- JSJessie S. JeonCorresponding
Massachusetts Institute of Technology
- SBSimone Bersini
Istituto Ortopedico Galeazzi, Istituti di Ricovero e Cura a Carattere Scientifico, Politecnico di Milano
- MGMara Gilardi
Istituto Ortopedico Galeazzi, Istituti di Ricovero e Cura a Carattere Scientifico, University of Milano-Bicocca
- GDGabriele Dubini
Politecnico di Milano
- JLJoseph L. Charest
Draper Laboratory
Topics & keywords
- Extravasation
- Tumor microenvironment
- Human breast
- Cancer
- Metastasis
- Biology
- Cancer cell
- Microfluidics
- Good health and well-being