Clathrin-independent endocytosis of ubiquitinated cargos
University of Milan · IFOM · +3 more institutions
Abstract
Plasma membrane receptors can be endocytosed through clathrin-dependent and clathrin-independent pathways. Here, we show that the epidermal growth factor (EGF) receptor (EGFR), when stimulated with low doses of EGF, is internalized almost exclusively through the clathrin pathway, and it is not ubiquitinated. At higher concentrations of ligand, however, a substantial fraction of the receptor is endocytosed through a clathrin-independent, lipid raft-dependent route, as the receptor becomes ubiquitinated. An ubiquitination-impaired EGFR mutant was internalized through the clathrin pathway, whereas an EGFR/ubiquitin chimera, that can signal solely through its ubiquitin (Ub) moiety, was internalized exclusively by…
Citation impact
- FWCI
- 28.59
- Percentile
- 100%
- References
- 29
Authors
8- SSSara SigismundCorresponding
University of Milan, IFOM, European Institute of Oncology, University of Genoa
- TWTanja Woelk
University of Milan, IFOM, European Institute of Oncology, University of Genoa
- CPClaudia Puri
University of Milan, IFOM, European Institute of Oncology, University of Genoa
- EMElena Maspero
University of Milan, IFOM, European Institute of Oncology, University of Genoa
- CTCarlo Tacchetti
University of Milan, IFOM, IRCCS Ospedale San Raffaele, European Institute of Oncology, University of Genoa
Topics & keywords
- Clathrin
- Internalization
- Endocytosis
- Ubiquitin
- Cell biology
- Signal transducing adaptor protein
- Receptor-mediated endocytosis
- Lipid raft