Assessing the performance of the Oxford Nanopore Technologies MinION

The Oxford Nanopore Technologies (ONT) MinION is a new sequencing technology that potentially offers read lengths of tens of kilobases (kb) limited only by the length of DNA molecules presented to it. The device has a low capital cost, is by far the most portable DNA sequencer available, and can produce data in real-time. It has numerous prospective applications including improving genome sequence assemblies and resolution of repeat-rich regions. Before such a technology is widely adopted, it is important to assess its performance and limitations in respect of throughput and accuracy. In this study we assessed the performance of the MinION by re-sequencing three bacterial genomes, with very different…

• WT
  Wellcome Trust

  Awards: WT101650MA, WT097835MF
• ON
  Oxford Nanopore Technologies
• UO
  University of Exeter
• BA
  Biotechnology and Biological Sciences Research Council

  Awards: BB/F017367/1, WT101650MA, BB/K003240/2, BB/K003240, BB/H016120/1, BB/K003240/1, BB/K003240/1, WT097835MF