A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies

A desirable but as yet unachieved property of a human immunodeficiency virus type 1 (HIV-1) vaccine candidate is the ability to induce broadly neutralizing antibodies (bNAbs). One approach to the problem is to create trimeric mimics of the native envelope glycoprotein (Env) spike that expose as many bNAb epitopes as possible, while occluding those for non-neutralizing antibodies (non-NAbs). Here, we describe the design and properties of soluble, cleaved SOSIP.664 gp140 trimers based on the subtype A transmitted/founder strain, BG505. These trimers are highly stable, more so even than the corresponding gp120 monomer, as judged by differential scanning calorimetry. They are also homogenous and closely resemble…

• IA
  International AIDS Vaccine Initiative

  Award: P01 AI82362
• AF
  Aids Fonds

  Award: 2012041
• NO
  Nederlandse Organisatie voor Wetenschappelijk Onderzoek
• NI
  National Institutes of Health

  Awards: P01 AI82362, AI36082, R37 AI36082
• CI
  Canadian Institutes of Health Research
• NC
  National Center for Research Resources

  Award: RR017573