Screening of Plant Extracts for Antioxidant Activity: a Comparative Study on Three Testing Methods
Wageningen University & Research · Bulgarian Academy of Sciences
Abstract
Three methods widely employed in the evaluation of antioxidant activity, namely 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, static headspace gas chromatography (HS-GC) and beta-carotene bleaching test (BCBT), have been compared with regard to their application in the screening of plant extracts. The strengths and limitations of each method have been illustrated by testing a number of extracts, of differing polarity, from plants of the genus Sideritis, and two known antioxidants (butylated hydroxytoluene and rosmarinic acid). The sample polarity was important for the exhibited activity in the BCBT and HS-GC methods but not for the DPPH method. The complex composition of the extracts and…
Citation impact
- FWCI
- 6.41
- Percentile
- 100%
- References
- 61
Authors
5Topics & keywords
- Chemistry
- DPPH
- Butylated hydroxytoluene
- Chromatography
- Antioxidant
- Polarity (international relations)
- Rosmarinic acid
- Gas chromatography