Multiplexed Toxin Analysis Using Four Colors of Quantum Dot Fluororeagents
Optical Sciences (United States) · Naval Research Laboratory Center for Bio/Molecular Science and Engineering · +1 more institution
Abstract
Quantum dots (QDs) have the potential to simplify the performance of multiplexed analysis. In this work, we prepared bioinorganic conjugates made with highly luminescent semiconductor nanocrystals (CdSe-ZnS core-shell QDs) and antibodies to perform multiplexed fluoroimmunoassays. Sandwich immunoassays for the detection of cholera toxin, ricin, shiga-like toxin 1, and staphylococcal enterotoxin B were performed simultaneously in single wells of a microtiter plate. Initially the assay performance for the detection of each toxin was examined. We then demonstrated the simultaneous detection of the four toxins from a single sample probed with a mixture of all four QD-antibody reagents. Using a simple linear…
Citation impact
- FWCI
- 25.85
- Percentile
- 100%
- References
- 22
Authors
7- EREllen R. GoldmanCorresponding
Optical Sciences (United States), Naval Research Laboratory Center for Bio/Molecular Science and Engineering, United States Naval Research Laboratory
- ARAaron R. Clapp
Naval Research Laboratory Center for Bio/Molecular Science and Engineering, Optical Sciences (United States), United States Naval Research Laboratory
- GPGeorge P. Anderson
United States Naval Research Laboratory, Naval Research Laboratory Center for Bio/Molecular Science and Engineering, Optical Sciences (United States)
- HTH. Tetsuo Uyeda
Naval Research Laboratory Center for Bio/Molecular Science and Engineering, United States Naval Research Laboratory, Optical Sciences (United States)
- JMJ. Matthew Mauro
United States Naval Research Laboratory, Naval Research Laboratory Center for Bio/Molecular Science and Engineering, Optical Sciences (United States)
Topics & keywords
- Chemistry
- Quantum dot
- Cholera toxin
- Toxin
- Enterotoxin
- Ricin
- Reagent
- Multiplexing