A Syntaxin 1, Gα o , and N-Type Calcium Channel Complex at a Presynaptic Nerve Terminal: Analysis by Quantitative Immunocolocalization
University Health Network · Toronto Western Hospital · +1 more institution
Abstract
Presynaptic Ca(V)2.2 (N-type) calcium channels are subject to modulation by interaction with syntaxin 1 and by a syntaxin 1-sensitive Galpha(O) G-protein pathway. We used biochemical analysis of neuronal tissue lysates and a new quantitative test of colocalization by intensity correlation analysis at the giant calyx-type presynaptic terminal of the chick ciliary ganglion to explore the association of Ca(V)2.2 with syntaxin 1 and Galpha(O). Ca(V)2.2 could be localized by immunocytochemistry (antibody Ab571) in puncta on the release site aspect of the presynaptic terminal and close to synaptic vesicle clouds. Syntaxin 1 coimmunoprecipitated with Ca(V)2.2 from chick brain and chick ciliary ganglia and was widely…
Citation impact
- FWCI
- 4.41
- Percentile
- 100%
- References
- 45
Authors
6- QLQi Li
University Health Network
- AGAnthony G. Lau
University Health Network, Toronto Western Hospital, Case Western Reserve University
- TJTerence J. Morris
University Health Network, Toronto Western Hospital, Case Western Reserve University
- LGLin Guo
University Health Network, Toronto Western Hospital, Case Western Reserve University
- CBChristopher B. Fordyce
University Health Network, Toronto Western Hospital, Case Western Reserve University
Topics & keywords
- Syntaxin
- Cell biology
- Biology
- STX1A
- Syntaxin 3
- Synaptotagmin 1
- Synaptic vesicle
- Biophysics