Genomic Analysis of Reactive Astrogliosis

Reactive astrogliosis is characterized by a profound change in astrocyte phenotype in response to all CNS injuries and diseases. To better understand the reactive astrocyte state, we used Affymetrix GeneChip arrays to profile gene expression in populations of reactive astrocytes isolated at various time points after induction using two mouse injury models, ischemic stroke and neuroinflammation. We find reactive gliosis consists of a rapid, but quickly attenuated, induction of gene expression after insult and identify induced Lcn2 and Serpina3n as strong markers of reactive astrocytes. Strikingly, reactive astrocyte phenotype strongly depended on the type of inducing injury. Although there is a core set of…

• CA
  Christopher and Dana Reeve Foundation
• DM
  Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
• MR
  Myelin Repair Foundation
• NI
  National Institutes of Health

  Award: GM49831