reviewLeukemiaSep 1, 2012HYBRID OA

EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols

Charles University · Universidad de Salamanca · +12 more institutions

PubMed
Indexed incrossrefpubmed

Abstract

The EU-supported EuroFlow Consortium aimed at innovation and standardization of immunophenotyping for diagnosis and classification of hematological malignancies by introducing 8-color flow cytometry with fully standardized laboratory procedures and antibody panels in order to achieve maximally comparable results among different laboratories. This required the selection of optimal combinations of compatible fluorochromes and the design and evaluation of adequate standard operating procedures (SOPs) for instrument setup, fluorescence compensation and sample preparation. Additionally, we developed software tools for the evaluation of individual antibody reagents and antibody panels. Each section describes what…

Citation impact

758
total citations
FWCI
14.78
Percentile
100%
References
65
Citations per year

Authors

21

Topics & keywords

Keywords
  • Immunophenotyping
  • Standardization
  • Medicine
  • Medical physics
  • Computer science
  • Pathology
  • Standard operating procedure
  • Software
UN Sustainable Development Goals
  • Industry, innovation and infrastructure
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