Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis
John Radcliffe Hospital · University of Oxford
Abstract
Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.
Citation impact
- FWCI
- 14.96
- Percentile
- 100%
- References
- 17
Authors
5- CGChris GardinerCorresponding
John Radcliffe Hospital, University of Oxford
- YJYannick J. Ferreira
John Radcliffe Hospital, University of Oxford
- RDRebecca Dragovic
John Radcliffe Hospital, University of Oxford
- CWChristopher W.G. Redman
John Radcliffe Hospital, University of Oxford
- ILIan L. Sargent
John Radcliffe Hospital, University of Oxford
Topics & keywords
- Nanoparticle tracking analysis
- Extracellular vesicles
- Enumeration
- Sizing
- Extracellular vesicle
- Protocol (science)
- Microvesicles
- Computer science