articlePLoS ONEJul 20, 2011GOLD OA

Prospective Genomic Characterization of the German Enterohemorrhagic Escherichia coli O104:H4 Outbreak by Rapid Next Generation Sequencing Technology

AMAlexander MellmannDHDag HarmsenCCCraig CummingsEBEmily B. ZentzSRShana R. Leopold

University of Münster · OpGen (United States) · +1 more institution

PubMed
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Abstract

An ongoing outbreak of exceptionally virulent Shiga toxin (Stx)-producing Escherichia coli O104:H4 centered in Germany, has caused over 830 cases of hemolytic uremic syndrome (HUS) and 46 deaths since May 2011. Serotype O104:H4, which has not been detected in animals, has rarely been associated with HUS in the past. To prospectively elucidate the unique characteristics of this strain in the early stages of this outbreak, we applied whole genome sequencing on the Life Technologies Ion Torrent PGM™ sequencer and Optical Mapping to characterize one outbreak isolate (LB226692) and a historic O104:H4 HUS isolate from 2001 (01-09591). Reference guided draft assemblies of both strains were completed with the newly…

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Topics & keywords

Topics
Keywords
  • Outbreak
  • Biology
  • Plasmid
  • Escherichia coli
  • Virulence
  • Microbiology
  • Serotype
  • Whole genome sequencing
UN Sustainable Development Goals
  • Responsible consumption and production
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