Membrane-Bound IL-21 Promotes Sustained Ex Vivo Proliferation of Human Natural Killer Cells
The University of Texas MD Anderson Cancer Center · The University of Texas Health Science Center at Houston
Abstract
NK cells have therapeutic potential for a wide variety of human malignancies. However, because NK cells expand poorly in vitro, have limited life spans in vivo, and represent a small fraction of peripheral white blood cells, obtaining sufficient cell numbers is the major obstacle for NK-cell immunotherapy. Genetically-engineered artificial antigen-presenting cells (aAPCs) expressing membrane-bound IL-15 (mbIL15) have been used to propagate clinical-grade NK cells for human trials of adoptive immunotherapy, but ex vivo proliferation has been limited by telomere shortening. We developed K562-based aAPCs with membrane-bound IL-21 (mbIL21) and assessed their ability to support human NK-cell proliferation. In…
Citation impact
- FWCI
- 13.73
- Percentile
- 100%
- References
- 102
Authors
13- CJCecele J. Denman
The University of Texas MD Anderson Cancer Center
- VVV. V. Senyukov
The University of Texas MD Anderson Cancer Center
- SSSrinivas S. Somanchi
The University of Texas MD Anderson Cancer Center
- PVPrasad V. Phatarpekar
The University of Texas Health Science Center at Houston, The University of Texas MD Anderson Cancer Center
- LMLisa M. Kopp
The University of Texas MD Anderson Cancer Center
Topics & keywords
- Interleukin 21
- Biology
- NKG2D
- Cell biology
- Interleukin 12
- Janus kinase 3
- Immunology
- Interleukin 15