A time- and cost-efficient system for high-level protein production in mammalian cells
Centre for Human Genetics · Cancer Research UK · +1 more institution
Abstract
Most proteins for structural biology studies are produced by high-level expression in Escherichia coli. However, prokaryotic based expression systems fail to generate correctly folded functional forms of many proteins and hence a variety of eukaryotic based expression systems have been developed. Of these, yeast and baculovirus-infected insect cells currently represent the expression systems of choice for structural biologists. Here, protocols for a simple, fast and affordable method for transient protein expression in mammalian cells are reported. The results demonstrate that it combines several features necessary for the production of suitable samples for structural biology, in particular protein…
Citation impact
- FWCI
- 5.32
- Percentile
- 100%
- References
- 11
Authors
3Topics & keywords
- Synthetic biology
- Computational biology
- Structural biology
- Escherichia coli
- Pipeline (software)
- Protein expression
- Protein biosynthesis
- Systems biology