Distinct and complex bacterial profiles in human periodontitis and health revealed by 16S pyrosequencing

Periodontitis has a polymicrobial etiology within the framework of a complex microbial ecosystem. With advances in sequencing technologies, comprehensive studies to elucidate bacterial community differences have recently become possible. We used 454 sequencing of 16S rRNA genes to compare subgingival bacterial communities from 29 periodontally healthy controls and 29 subjects with chronic periodontitis. Amplicons from both the V1-2 and V4 regions of the 16S gene were sequenced, yielding 1,393,579 sequences. They were identified by BLAST against a curated oral 16S database, and mapped to 16 phyla, 106 genera, and 596 species. 81% of sequences could be mapped to cultivated species. Differences between health-…

• UD
  U.S. Department of Energy

  Awards: AC05-00OR22725, DE-AC05, 00OR22725
• B
  Battelle

  Awards: DE-AC05, DE-AC05-00OR22725
• U
  UT-Battelle

  Awards: DE-AC05-, AC05-00OR22725
• OO
  Office of Science

  Awards: DE-AC05-00OR22725, AC05-00OR22725
• NI
  National Institute of Dental and Craniofacial Research
• BA
  Biological and Environmental Research

  Awards: DE-AC05-00OR22725, 00OR22725
• OR
  Oak Ridge National Laboratory

  Award: AC05-00OR22725