Chemiexcitation of melanin derivatives induces DNA photoproducts long after UV exposure

Mutations in sunlight-induced melanoma arise from cyclobutane pyrimidine dimers (CPDs), DNA photoproducts that are typically created picoseconds after an ultraviolet (UV) photon is absorbed at thymine or cytosine. We found that in melanocytes, CPDs are generated for >3 hours after exposure to UVA, a major component of the radiation in sunlight and in tanning beds. These "dark CPDs" constitute the majority of CPDs and include the cytosine-containing CPDs that initiate UV-signature C→T mutations. Dark CPDs arise when UV-induced reactive oxygen and nitrogen species combine to excite an electron in fragments of the pigment melanin. This creates a quantum triplet state that has the energy of a UV photon but induces…

• FD
  Fundação de Amparo à Pesquisa do Estado de São Paulo

  Award: 06/56530-4
• VU
  Veterinärmedizinische Universität Wien
• NI
  National Institutes of Health

  Awards: P50 CA121974, P30 DK34989