Detergent binding explains anomalous SDS-PAGE migration of membrane proteins
University of Toronto · Hospital for Sick Children
Abstract
Migration on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) that does not correlate with formula molecular weights, termed "gel shifting," appears to be common for membrane proteins but has yet to be conclusively explained. In the present work, we investigate the anomalous gel mobility of helical membrane proteins using a library of wild-type and mutant helix-loop-helix ("hairpin") sequences derived from transmembrane segments 3 and 4 of the human cystic fibrosis transmembrane conductance regulator (CFTR), including disease-phenotypic residue substitutions. We find that these hairpins migrate at rates of -10% to +30% vs. their actual formula weights on SDS-PAGE and load detergent at…
Citation impact
- FWCI
- 20.94
- Percentile
- 100%
- References
- 58
Authors
5- ARArianna RathCorresponding
University of Toronto, Hospital for Sick Children
- MGMira Glibowicka
University of Toronto, Hospital for Sick Children
- VGVincent G. Nadeau
University of Toronto, Hospital for Sick Children
- GCGong Chen
University of Toronto, Hospital for Sick Children
- CMCharles M. Deber
University of Toronto, Hospital for Sick Children
Topics & keywords
- Gel electrophoresis
- Polyacrylamide gel electrophoresis
- Membrane protein
- Transmembrane protein
- Mutant
- Cystic fibrosis transmembrane conductance regulator
- Transmembrane domain
- Chemistry