MicroRNAs control translation initiation by inhibiting eukaryotic initiation factor 4E/cap and poly(A) tail function
Victor Chang Cardiac Research Institute · UNSW Sydney · +1 more institution
Abstract
MicroRNAs (miRNAs) repress translation of target mRNAs by interaction with partially mismatched sequences in their 3' UTR. The mechanism by which they act on translation has remained largely obscure. We examined the translation of mRNAs containing four partially mismatched miRNA-binding sites in the 3' UTR in HeLa cells cotransfected with a cognate miRNA. The mRNAs were prepared by in vitro transcription and were engineered to employ different modes of translation initiation. We find that the 5' cap structure and the 3' poly(A) tail are each necessary but not sufficient for full miRNA-mediated repression of mRNA translation. Replacing the cap structure with an internal ribosome entry site from either the…
Citation impact
- FWCI
- 18.32
- Percentile
- 100%
- References
- 54
Authors
4- DTDavid T. Humphreys
Victor Chang Cardiac Research Institute, UNSW Sydney, St Vincent's Clinic
- BJBelinda J. Westman
Victor Chang Cardiac Research Institute, UNSW Sydney, St Vincent's Clinic
- DIDavid I. K. Martin
Victor Chang Cardiac Research Institute, UNSW Sydney, St Vincent's Clinic
- TPThomas PreißCorresponding
Victor Chang Cardiac Research Institute, UNSW Sydney, St Vincent's Clinic
Topics & keywords
- Internal ribosome entry site
- Eukaryotic initiation factor
- Translation (biology)
- Eukaryotic translation
- Eukaryotic translation initiation factor 4 gamma
- Biology
- EIF4E
- Five prime untranslated region