Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions

The recent rapid development of single-particle electron cryo-microscopy (cryo-EM) now allows structures to be solved by this method at resolutions close to 3 Å. Here, a number of tools to facilitate the interpretation of EM reconstructions with stereochemically reasonable all-atom models are described. The BALBES database has been repurposed as a tool for identifying protein folds from density maps. Modifications to Coot, including new Jiggle Fit and morphing tools and improved handling of nucleic acids, enhance its functionality for interpreting EM maps. REFMAC has been modified for optimal fitting of atomic models into EM maps. As external structural information can enhance the reliability of the derived…

• AI
  Agouron Institute
• WT
  Wellcome Trust

  Award: WT096570
• MR
  Medical Research Council

  Awards: MC_U105184332, MC_UP_A025_1012