articleMolecular Ecology ResourcesMay 9, 2015Closed access

obitools : a unix ‐inspired software package for DNA metabarcoding

Centre National de la Recherche Scientifique · Laboratoire d'Écologie Alpine · +2 more institutions

PubMed
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Abstract

DNA metabarcoding offers new perspectives in biodiversity research. This recently developed approach to ecosystem study relies heavily on the use of next-generation sequencing (NGS) and thus calls upon the ability to deal with huge sequence data sets. The obitools package satisfies this requirement thanks to a set of programs specifically designed for analysing NGS data in a DNA metabarcoding context. Their capacity to filter and edit sequences while taking into account taxonomic annotation helps to set up tailor-made analysis pipelines for a broad range of DNA metabarcoding applications, including biodiversity surveys or diet analyses. The obitools package is distributed as an open source software available…

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