articleStem CellsDec 1, 2005BRONZE OA

Immunophenotype of Human Adipose-Derived Cells: Temporal Changes in Stromal-Associated and Stem Cell–Associated Markers

JBJames B. MitchellKRKevin R. McIntoshSZSanjin ZvonicSMSara M. GarrettZEZ. Elizabeth Floyd

Pennington Biomedical Research Center · Sepragen Corporation (United States) · +1 more institution

PubMed
Indexed incrossrefpubmed

Abstract

Adipose tissue represents an abundant and accessible source of multipotent adult stem cells and is used by many investigators for tissue engineering applications; however, not all laboratories use cells at equivalent stages of isolation and passage. We have compared the immunophenotype of freshly isolated human adipose tissue-derived stromal vascular fraction (SVF) cells relative to serial-passaged adipose-derived stem cells (ASCs). The initial SVF cells contained colony-forming unit fibroblasts at a frequency of 1:32. Colony-forming unit adipocytes and osteoblasts were present in the SVF cells at comparable frequencies (1:28 and 1:16, respectively). The immunophenotype of the adipose-derived cells based on…

Citation impact

1,116
total citations
FWCI
12.35
Percentile
100%
References
65
Citations per year

Authors

12

Topics & keywords

Topics
Keywords
  • Biology
  • Adipose tissue
  • Stem cell
  • Stromal vascular fraction
  • CD90
  • Immunophenotyping
  • CD34
  • Stem cell marker
No related works found for this paper.

Funding