articleBioinformaticsMar 22, 2007HYBRID OA

Enhancements and modifications of primer design program Primer3

TKTriinu KõressaarMRMaido Remm

University of Tartu

PubMed
Indexed incrossrefdoajpubmed

Abstract

UNLABELLED: The determination of annealing temperature is a critical step in PCR design. This parameter is typically derived from the melting temperature of the PCR primers, so for successful PCR work it is important to determine the melting temperature of primer accurately. We introduced several enhancements in the widely used primer design program Primer3. The improvements include a formula for calculating melting temperature and a salt correction formula. Also, the new version can take into account the effects of divalent cations, which are included in most PCR buffers. Another modification enables using lowercase masked template sequences for primer design. AVAILABILITY: Features described in this article…

Citation impact

2,694
total citations
FWCI
3.63
Percentile
100%
References
15
Citations per year

Authors

2

Topics & keywords

Topics
Keywords
  • Primer (cosmetics)
  • Computer science
  • Melting temperature
  • Code (set theory)
  • Programming language
  • Thermodynamics
  • Set (abstract data type)
  • Materials science
No related works found for this paper.

Funding