Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics
Howard Hughes Medical Institute · Janelia Research Campus · +8 more institutions
Abstract
Super-resolution fluorescence microscopy is distinct among nanoscale imaging tools in its ability to image protein dynamics in living cells. Structured illumination microscopy (SIM) stands out in this regard because of its high speed and low illumination intensities, but typically offers only a twofold resolution gain. We extended the resolution of live-cell SIM through two approaches: ultrahigh numerical aperture SIM at 84-nanometer lateral resolution for more than 100 multicolor frames, and nonlinear SIM with patterned activation at 45- to 62-nanometer resolution for approximately 20 to 40 frames. We applied these approaches to image dynamics near the plasma membrane of spatially resolved assemblies of…
Citation impact
- FWCI
- 109.93
- Percentile
- 100%
- References
- 67
Authors
15- DLDong Li
Howard Hughes Medical Institute, Janelia Research Campus
- LSLin Shao
Howard Hughes Medical Institute, Janelia Research Campus
- BCBi‐Chang Chen
Howard Hughes Medical Institute, Janelia Research Campus
- XZXi Zhang
Central China Normal University, Institute of Biophysics
- MZMingshu Zhang
Institute of Biophysics
Topics & keywords
- Resolution (logic)
- Cytoskeleton
- Endocytic cycle
- Endocytosis
- Superresolution
- Live cell imaging
- Microscopy
- Temporal resolution