MAFFT version 5: improvement in accuracy of multiple sequence alignment
Kyoto University · Kyoto University Institute for Chemical Research
Abstract
The accuracy of multiple sequence alignment program MAFFT has been improved. The new version (5.3) of MAFFT offers new iterative refinement options, H-INS-i, F-INS-i and G-INS-i, in which pairwise alignment information are incorporated into objective function. These new options of MAFFT showed higher accuracy than currently available methods including TCoffee version 2 and CLUSTAL W in benchmark tests consisting of alignments of >50 sequences. Like the previously available options, the new options of MAFFT can handle hundreds of sequences on a standard desktop computer. We also examined the effect of the number of homologues included in an alignment. For a multiple alignment consisting of approximately 8…
Citation impact
- FWCI
- 44.47
- Percentile
- 100%
- References
- 37
Authors
1Topics & keywords
- Multiple sequence alignment
- Benchmark (surveying)
- Sequence alignment
- Pairwise comparison
- Sequence (biology)
- Biology
- Similarity (geometry)
- Computer science