Bruchpilot Promotes Active Zone Assembly, Ca 2+ Channel Clustering, and Vesicle Release
University of Würzburg · Ludwig Boltzmann für Klinische Neurobiologie · +2 more institutions
Abstract
The molecular organization of presynaptic active zones during calcium influx-triggered neurotransmitter release is the focus of intense investigation. The Drosophila coiled-coil domain protein Bruchpilot (BRP) was observed in donut-shaped structures centered at active zones of neuromuscular synapses by using subdiffraction resolution STED (stimulated emission depletion) fluorescence microscopy. At brp mutant active zones, electron-dense projections (T-bars) were entirely lost, Ca2+ channels were reduced in density, evoked vesicle release was depressed, and short-term plasticity was altered. BRP-like proteins seem to establish proximity between Ca2+ channels and vesicles to allow efficient transmitter release…
Citation impact
- FWCI
- 17.13
- Percentile
- 100%
- References
- 39
Authors
14- RJRobert J. KittelCorresponding
University of Würzburg, Ludwig Boltzmann für Klinische Neurobiologie, European Neuroscience Institute Göttingen, Max Planck Institute for Biophysical Chemistry
- CWCarolin Wichmann
University of Würzburg, Ludwig Boltzmann für Klinische Neurobiologie, European Neuroscience Institute Göttingen, Max Planck Institute for Biophysical Chemistry
- TMTobias M. Rasse
University of Würzburg, Ludwig Boltzmann für Klinische Neurobiologie, European Neuroscience Institute Göttingen, Max Planck Institute for Biophysical Chemistry
- WFWernher Fouquet
University of Würzburg, Ludwig Boltzmann für Klinische Neurobiologie, European Neuroscience Institute Göttingen, Max Planck Institute for Biophysical Chemistry
- MSManuela Schmidt
University of Würzburg, Ludwig Boltzmann für Klinische Neurobiologie, European Neuroscience Institute Göttingen, Max Planck Institute for Biophysical Chemistry
Topics & keywords
- Active zone
- Vesicle
- Biophysics
- Synaptic vesicle
- Chemistry
- Neurotransmitter
- Ribbon synapse
- Cell biology