Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system
Whitehead Institute for Biomedical Research · Massachusetts Institute of Technology · +1 more institution
Abstract
Technologies allowing for specific regulation of endogenous genes are valuable for the study of gene functions and have great potential in therapeutics. We created the CRISPR-on system, a two-component transcriptional activator consisting of a nuclease-dead Cas9 (dCas9) protein fused with a transcriptional activation domain and single guide RNAs (sgRNAs) with complementary sequence to gene promoters. We demonstrate that CRISPR-on can efficiently activate exogenous reporter genes in both human and mouse cells in a tunable manner. In addition, we show that robust reporter gene activation in vivo can be achieved by injecting the system components into mouse zygotes. Furthermore, we show that CRISPR-on can…
Citation impact
- FWCI
- 35.75
- Percentile
- 100%
- References
- 27
Authors
10- AWAlbert W. ChengCorresponding
Whitehead Institute for Biomedical Research
- HWHaoyi Wang
Whitehead Institute for Biomedical Research
- HYHui Yang
Whitehead Institute for Biomedical Research
- LSLinyu Shi
Whitehead Institute for Biomedical Research
- YKYarden Katz
Massachusetts Institute of Technology, Institute of Cognitive and Brain Sciences, Whitehead Institute for Biomedical Research
Topics & keywords
- Biology
- CRISPR
- Gene
- Guide RNA
- Promoter
- Cas9
- Reporter gene
- Activator (genetics)