articleAngewandte Chemie International EditionJul 26, 2005Closed access

Label‐Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer‐Based Sensor

YXYi XiaoAAArica A. LubinAJAlan J. HeegerKWKevin W. Plaxco

University of California, Santa Barbara

PubMed
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Abstract

Blue, gold, and DNA: A methylene blue (MB) tagged, thrombin-binding DNA aptamer immobilized on a gold surface undergoes a large conformational change upon target binding (see schematic representation; eT: electron transfer). This folding produces a large, readily measurable change in redox current and allows the electrochemical detection of thrombin in blood serum.

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Topics & keywords

Topics
Keywords
  • Aptamer
  • Thrombin
  • Methylene blue
  • Chemistry
  • Biosensor
  • DNA
  • Conformational change
  • Biophysics
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