Universal primer cocktails for fish DNA barcoding

Abstract Reliable recovery of the 5′ region of the cytochrome c oxidase 1 (COI) gene is critical for the ongoing effort to gather DNA barcodes for all fish species. In this study, we develop and test primer cocktails with a view towards increasing the efficiency of barcode recovery. Specifically, we evaluate the success of polymerase chain reaction amplification and the quality of resultant sequences using three primer cocktails on DNA extracts from representatives of 94 fish families. Our results show that M13‐tailed primer cocktails are more effective than conventional degenerate primers, allowing barcode work on taxonomically diverse samples to be carried out in a high‐throughput fashion.

• GA
  Gordon and Betty Moore Foundation
• OI
  Ontario Innovation Trust
• GC
  Genome Canada
• OG
  Ontario Genomics Institute
• CR
  Canada Research Chairs
• NS
  Natural Sciences and Engineering Research Council of Canada