Regulated Ire1-dependent decay of messenger RNAs in mammalian cells
Howard Hughes Medical Institute · University of California, San Francisco · +1 more institution
Abstract
Maintenance of endoplasmic reticulum (ER) function is achieved in part through Ire1 (inositol-requiring enzyme 1), a transmembrane protein activated by protein misfolding in the ER. The cytoplasmic nuclease domain of Ire1 cleaves the messenger RNA (mRNA) encoding XBP-1 (X-box-binding protein 1), enabling splicing and production of this active transcription factor. We recently showed that Ire1 activation independently induces the rapid turnover of mRNAs encoding membrane and secreted proteins in Drosophila melanogaster cells through a pathway we call regulated Ire1-dependent decay (RIDD). In this study, we show that mouse fibroblasts expressing wild-type Ire1 but not an Ire1 variant lacking nuclease activity…
Citation impact
- FWCI
- 20.07
- Percentile
- 100%
- References
- 31
Authors
6- JHJulie HollienCorresponding
Howard Hughes Medical Institute, University of California, San Francisco, University of Utah
- JHJonathan H. Lin
Howard Hughes Medical Institute, University of California, San Francisco
- HLHan Li
Howard Hughes Medical Institute, University of California, San Francisco
- NSNicole Stevens
University of Utah
- PWPeter Walter
Howard Hughes Medical Institute, University of California, San Francisco
Topics & keywords
- Endoplasmic reticulum
- Biology
- RNA splicing
- Unfolded protein response
- Cell biology
- Messenger RNA
- Nuclease
- XBP1