Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform

With read lengths of currently up to 2 × 300 bp, high throughput and low sequencing costs Illumina's MiSeq is becoming one of the most utilized sequencing platforms worldwide. The platform is manageable and affordable even for smaller labs. This enables quick turnaround on a broad range of applications such as targeted gene sequencing, metagenomics, small genome sequencing and clinical molecular diagnostics. However, Illumina error profiles are still poorly understood and programs are therefore not designed for the idiosyncrasies of Illumina data. A better knowledge of the error patterns is essential for sequence analysis and vital if we are to draw valid conclusions. Studying true genetic variation in a…

• SR
  Sight Research UK

  Award: NE/L011956/1
• MR
  Medical Research Council

  Award: MR/K002279/1
• EA
  Engineering and Physical Sciences Research Council

  Awards: EP/H003851/1, EP/F007868/1, EP/K038885/1, EP/H003851/1
• BA
  Biotechnology and Biological Sciences Research Council

  Awards: BB/I004416/1, BB/L014777/1, BB/I02347X/1
• NE
  Natural Environment Research Council

  Award: NE/L011956/1