Small dsRNAs induce transcriptional activation in human cells
San Francisco VA Medical Center · University of California, San Francisco · +1 more institution
Abstract
Recent studies have shown that small noncoding RNAs, such as microRNAs and siRNAs, regulate gene expression at multiple levels including chromatin architecture, transcription, RNA editing, RNA stability, and translation. Each form of RNA-dependent regulation has been generally found to silence homologous sequences and collectively called RNAi. To further study the regulatory role of small RNAs at the transcriptional level, we designed and synthesized 21-nt dsRNAs targeting selected promoter regions of human genes E-cadherin, p21(WAF1/CIP1) (p21), and VEGF. Surprisingly, transfection of these dsRNAs into human cell lines caused long-lasting and sequence-specific induction of targeted genes. dsRNA mutation…
Citation impact
- FWCI
- 16.08
- Percentile
- 100%
- References
- 37
Authors
8- LLLong‐Cheng LiCorresponding
San Francisco VA Medical Center, University of California, San Francisco
- STSteven T. Okino
San Francisco VA Medical Center, University of California, San Francisco, Fred Hutch Cancer Center
- HZHong Zhao
San Francisco VA Medical Center, University of California, San Francisco, Fred Hutch Cancer Center
- DPDeepa Pookot
San Francisco VA Medical Center, University of California, San Francisco, Fred Hutch Cancer Center
- RFRobert F. Place
San Francisco VA Medical Center, University of California, San Francisco, Fred Hutch Cancer Center
Topics & keywords
- RNA silencing
- Biology
- Argonaute
- Gene silencing
- RNA
- Trans-acting siRNA
- RNA interference
- Non-coding RNA