Genome-wide analysis of cAMP-response element binding protein occupancy, phosphorylation, and target gene activation in human tissues

Zhang, Xinmin; Odom, Duncan T.; Koo, Seung‐Hoi; Conkright, Michael D.; Canettieri, Gianluca; Best, Jennifer L.; Chen, Huaming; Jenner, Richard G.; Herbolsheimer, Elizabeth; Jacobsen, Elizabeth A.; Kadam, Shilpa D.; Ecker, Joseph R.; Emerson, Beverly M.; Hogenesch, John B.; Unterman, Terry G.; Young, Richard A.; Montminy, Marc

doi:10.1073/pnas.0501076102

articleProceedings of the National Academy of SciencesMar 7, 2005Closed access

Genome-wide analysis of cAMP-response element binding protein occupancy, phosphorylation, and target gene activation in human tissues

XZXinmin Zhang DTDuncan T. Odom SKSeung‐Hoi Koo MDMichael D. Conkright GCGianluca Canettieri

Genomics Institute of the Novartis Research Foundation · Salk Institute for Biological Studies · +2 more institutions

PubMed

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Abstract

Hormones and nutrients often induce genetic programs via signaling pathways that interface with gene-specific activators. Activation of the cAMP pathway, for example, stimulates cellular gene expression by means of the PKA-mediated phosphorylation of cAMP-response element binding protein (CREB) at Ser-133. Here, we use genome-wide approaches to characterize target genes that are regulated by CREB in different cellular contexts. CREB was found to occupy approximately 4,000 promoter sites in vivo, depending on the presence and methylation state of consensus cAMP response elements near the promoter. The profiles for CREB occupancy were very similar in different human tissues, and exposure to a cAMP agonist…

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Topics

Keywords

CREB
CREB1
Coactivator
Phosphorylation
Biology
Cyclic AMP Response Element-Binding Protein
Response element
Promoter

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