Eleven Golden Rules of Quantitative RT-PCR
Max Planck Society · Max Planck Institute of Molecular Plant Physiology · +2 more institutions
Indexed incrossrefpubmed
Abstract
Reverse transcription followed by quantitative polymerase chain reaction analysis, or qRT-PCR, is an extremely sensitive, cost-effective method for quantifying gene transcripts from plant cells. The availability of nonspecific double-stranded DNA (dsDNA) binding fluorophors, such as SYBR Green, and
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712
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Authors
3- MKMichael K. UdvardiCorresponding
Max Planck Society, Max Planck Institute of Molecular Plant Physiology, Noble Research Institute
- TCTomasz Czechowski
Max Planck Society, Max Planck Institute of Molecular Plant Physiology, University of York
- WSWolf‐Rüdiger Scheible
Max Planck Society, Max Planck Institute of Molecular Plant Physiology
Topics & keywords
Topics
Keywords
- Biology
- Real-time polymerase chain reaction
- Computational biology
- Genetics
- Gene
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