Blood‐brain barrier‐specific properties of a human adult brain endothelial cell line

Establishment of a human model of the blood-brain barrier has proven to be a difficult goal. To accomplish this, normal human brain endothelial cells were transduced by lentiviral vectors incorporating human telomerase or SV40 T antigen. Among the many stable immortalized clones obtained by sequential limiting dilution cloning of the transduced cells, one was selected for expression of normal endothelial markers, including CD31, VE cadherin, and von Willebrand factor. This cell line, termed hCMEC/D3, showed a stable normal karyotype, maintained contact-inhibited monolayers in tissue culture, exhibited robust proliferation in response to endothelial growth factors, and formed capillary tubes in matrix but no…

• OS
  Open Society Foundations
• W
  Wellcome
• FP
  Fondation pour l'Aide à la Recherche sur la Sclérose en Plaques
• S
  Sidaction
• MS
  Multiple Sclerosis Society of Canada
• LT
  Leverhulme Trust
• IN
  Institut National de la Santé et de la Recherche Médicale
• CN
  Centre National de la Recherche Scientifique
• UP
  Université Paris Descartes
• NH
  National Heart, Lung, and Blood Institute