Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow
Keio University · Czech Academy of Sciences, Institute of Physiology · +3 more institutions
Abstract
Mesenchymal stem cells (MSCs) are defined as cells that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identified in vivo and little is known about their physiological roles or even their exact tissue location. In this study, we used phenotypic, morphological, and functional criteria to identify and prospectively isolate a subset of MSCs (PDGFRalpha+Sca-1+CD45-TER119-) from adult mouse bone marrow. Individual MSCs generated colonies at a high frequency and could differentiate into hematopoietic niche cells, osteoblasts, and adipocytes after in vivo transplantation. Naive MSCs…
Citation impact
- FWCI
- 19.50
- Percentile
- 100%
- References
- 60
Authors
16- SMSatoru Morikawa
Keio University, Czech Academy of Sciences, Institute of Physiology
- YMYo Mabuchi
Keio University, Czech Academy of Sciences, Institute of Physiology
- YKYoshiaki Kubota
Keio University, Czech Academy of Sciences, Institute of Physiology
- YNYasuo Nagai
Keio University, Czech Academy of Sciences, Institute of Physiology
- KNKunimichi Niibe
Keio University, Czech Academy of Sciences, Institute of Physiology
Topics & keywords
- Mesenchymal stem cell
- Clinical uses of mesenchymal stem cells
- Biology
- Bone marrow
- Stem cell transplantation for articular cartilage repair
- Transplantation
- Stem cell
- Haematopoiesis