Differential Contribution of Monocytes to Heart Macrophages in Steady-State and After Myocardial Infarction
Harvard University · Amsterdam UMC Location University of Amsterdam · +3 more institutions
Abstract
RATIONALE: Macrophages populate the steady-state myocardium. Previously, all macrophages were thought to arise from monocytes; however, it emerged that, in several organs, tissue-resident macrophages may self-maintain through local proliferation. OBJECTIVE: Our aim was to study the contribution of monocytes to cardiac-resident macrophages in steady state, after macrophage depletion in CD11b(DTR/+) mice and in myocardial infarction. METHODS AND RESULTS: Using in vivo fate mapping and flow cytometry, we estimated that during steady state the heart macrophage population turns over in ≈1 month. To explore the source of cardiac-resident macrophages, we joined the circulation of mice using parabiosis. After 6 weeks,…
Citation impact
- FWCI
- 19.97
- Percentile
- 100%
- References
- 21
Authors
13- THTimo HeidtCorresponding
Harvard University, Amsterdam UMC Location University of Amsterdam, Center for Systems Biology, Auburn University, University of Amsterdam
- GCGabriel Courties
Harvard University, Amsterdam UMC Location University of Amsterdam, Center for Systems Biology, Auburn University, University of Amsterdam
- PDPartha Dutta
Harvard University, Amsterdam UMC Location University of Amsterdam, Center for Systems Biology, Auburn University, University of Amsterdam
- HBHendrik B. Sager
Harvard University, Amsterdam UMC Location University of Amsterdam, Center for Systems Biology, Auburn University, University of Amsterdam
- MSMatt Sebas
Harvard University, Amsterdam UMC Location University of Amsterdam, Center for Systems Biology, Auburn University, University of Amsterdam
Topics & keywords
- Macrophage
- Myocardial infarction
- Monocyte
- Integrin alpha M
- Parabiosis
- Immunology
- Population
- Inflammation
- Good health and well-being