Stable metal-organic frameworks containing single-molecule traps for enzyme encapsulation

Enzymatic catalytic processes possess great potential in chemical manufacturing, including pharmaceuticals, fuel production and food processing. However, the engineering of enzymes is severely hampered due to their low operational stability and difficulty of reuse. Here, we develop a series of stable metal-organic frameworks with rationally designed ultra-large mesoporous cages as single-molecule traps (SMTs) for enzyme encapsulation. With a high concentration of mesoporous cages as SMTs, PCN-333(Al) encapsulates three enzymes with record-high loadings and recyclability. Immobilized enzymes that most likely undergo single-enzyme encapsulation (SEE) show smaller Km than free enzymes while maintaining comparable…

• UD
  U.S. Department of Energy

  Awards: DE-AR0000249, AC02-06CH11357, Contract No. DE-AC02-06CH11357, DE-AC02, 06CH11357, No. DE-AC02-06CH11357, DE-AC02-06CH11357, DE-AC02-
• V
  VINNOVA

  Award: 3DEM-NATUR
• V
  Vetenskapsrådet

  Award: DE-AC02-06CH11357
• OO
  Office of Science

  Awards: DE-AC02-06CH11357, Contract No. DE-AC02-06CH11357, DE-AC02, No. DE-AC02-06CH11357, 06CH11357, AC02-06CH11357
• OO
  Office of Naval Research

  Awards: DE-AC02-06CH11357, N000141310753
• BE
  Basic Energy Sciences

  Awards: DE-AC02, Contract No. DE-AC02-06CH11357, AC02-06CH11357, 06CH11357
• AN
  Argonne National Laboratory

  Awards: DE-AC02, No. DE-AC02-06CH11357, 06CH11357, AC02-06CH11357, Contract No. DE-AC02-06CH11357