Trypan Blue Exclusion Test of Cell Viability
National Institute of Allergy and Infectious Diseases
Indexed incrossrefpubmed
Abstract
The protocol described in this appendix allows for light microscopic quantitation of cell viability. Cells are suspended in PBS containing trypan blue and then examined to determine the percentage of cells that have clear cytoplasm (viable cells) versus cells that have blue cytoplasm (nonviable cells).
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1Topics & keywords
Topics
Keywords
- Trypan blue
- Cytoplasm
- Viability assay
- Cell
- Molecular biology
- Cell counting
- Biology
- Cell biology
UN Sustainable Development Goals
- Reduced inequalities
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