Expression of Helios, an Ikaros Transcription Factor Family Member, Differentiates Thymic-Derived from Peripherally Induced Foxp3+ T Regulatory Cells
Laboratory of Molecular Genetics · National Institute of Allergy and Infectious Diseases · +1 more institution
Abstract
Helios, a member of the Ikaros transcription factor family, is preferentially expressed at the mRNA level by regulatory T cells (Treg cells). We evaluated Helios protein expression using a newly generated mAb and demonstrated that it is expressed in all thymocytes at the double negative 2 stage of thymic development. Although Helios was expressed by 100% of CD4(+)CD8(-)Foxp3(+) thymocytes, its expression in peripheral lymphoid tissues was restricted to a subpopulation ( approximately 70%) of Foxp3(+) T cells in mice and humans. Neither mouse nor human naive T cells induced to express Foxp3 in vitro by TCR stimulation in the presence of TGF-beta expressed Helios. Ag-specific Foxp3(+) T cells induced in vivo by…
Citation impact
- FWCI
- 121.61
- Percentile
- 100%
- References
- 48
Authors
7- AMAngela M. ThorntonCorresponding
Laboratory of Molecular Genetics, National Institute of Allergy and Infectious Diseases
- PEPatricia E. Korty
Laboratory of Molecular Genetics
- DQDat Q. Tran
Laboratory of Molecular Genetics
- EAElizabeth A. Wohlfert
National Institutes of Health, National Institute of Allergy and Infectious Diseases
- PEPatrick E. Murray
Laboratory of Molecular Genetics
Topics & keywords
- Transcription factor
- FOXP3
- Cell biology
- Forkhead Transcription Factors
- Expression (computer science)
- Biology
- Genetics
- Gene