Targeting DNA Double-Strand Breaks with TAL Effector Nucleases
University of Minnesota · Iowa State University · +1 more institution
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Abstract
Engineered nucleases that cleave specific DNA sequences in vivo are valuable reagents for targeted mutagenesis. Here we report a new class of sequence-specific nucleases created by fusing transcription activator-like effectors (TALEs) to the catalytic domain of the FokI endonuclease. Both native and custom TALE-nuclease fusions direct DNA double-strand breaks to specific, targeted sites.
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8Topics & keywords
Topics
Keywords
- Transcription activator-like effector nuclease
- Biology
- Nuclease
- Genome editing
- Effector
- DNA
- Cleave
- Genetics
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