articleJournal of Clinical MicrobiologyOct 29, 2009GREEN OA

Rapid Detection of Mycobacterium tuberculosis and Rifampin Resistance by Use of On-Demand, Near-Patient Technology

DHDanica HelbMJMartin JonesESElizabeth Story‐RollerCBCatharina BoehmeEWEllen Wallace

Rutgers, The State University of New Jersey · Rutgers New Jersey Medical School · +3 more institutions

PubMed
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Abstract

Current nucleic acid amplification methods to detect Mycobacterium tuberculosis are complex, labor-intensive, and technically challenging. We developed and performed the first analysis of the Cepheid Gene Xpert System's MTB/RIF assay, an integrated hands-free sputum-processing and real-time PCR system with rapid on-demand, near-patient technology, to simultaneously detect M. tuberculosis and rifampin resistance. Analytic tests of M. tuberculosis DNA demonstrated a limit of detection (LOD) of 4.5 genomes per reaction. Studies using sputum spiked with known numbers of M. tuberculosis CFU predicted a clinical LOD of 131 CFU/ml. Killing studies showed that the assay's buffer decreased M. tuberculosis viability by…

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Topics & keywords

Topics
Keywords
  • Tuberculosis
  • Sputum
  • Mycobacterium tuberculosis
  • GeneXpert MTB/RIF
  • Microbiology
  • Mycobacterium tuberculosis complex
  • Medicine
  • Drug resistance
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